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04-23335 National Institute of Environmental Health Sciences (NIEHS); National Toxicology Program (NTP); NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM): Availability of Updated Standardized In Vitro Cytotoxicity Test Method Protocols for Estimating Acute Oral Systemic Toxicity; Request for Existing In Vivo and In Vitro Acute Toxicity Data Notice NICEATM announces the availability of two updated standardized in vitro cytotoxicity test method protocols to estimate acute oral systemic toxicity in rodents. These two test methods were previously recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) for selecting starting doses for in vivo acute oral systemic toxicity tests (Federal Register Vol. 66, No. 189, pages 49686-49687, September 28, 2001). This approach can reduce the number of animals required for acute oral toxicity testing. NICEATM also requests the submission of existing and future data on chemicals and products tested for both acute oral systemic toxicity and in vitro cytotoxicity using the standardized test method protocols mentioned in this notice. These data will be used to further evaluate the usefulness and limitations of cytotoxicity methods for estimating in vivo acute oral toxicity. The data will also be used to establish a database to support the investigation of other test methods necessary to improve the accuracy of in vitro assessments of acute systemic toxicity. 2004-10-19 2004 10 https://www.federalregister.gov/documents/2004/10/19/04-23335/national-institute-of-environmental-health-sciences-niehs-national-toxicology-program-ntp-ntp https://www.govinfo.gov/content/pkg/FR-2004-10-19/pdf/04-23335.pdf Health and Human Services Department; Public Health Service 221,442 NICEATM announces the availability of two updated standardized in vitro cytotoxicity test method protocols to estimate acute oral systemic toxicity in rodents. These two test methods were previously recommended by the Interagency Coordinating Committee...

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